21 Jun 2012 Retracing of Lgr5+ adenoma cells. (A) Apc-deficient adenomas were allowed to develop for 24 days in Lgr5-EGFP-Ires-CreERT2/Apcfl/fl/R26R

Lgr5 EGFP‐Ires‐CreERT2 knock‐in mice (Barker et al., 2007) and Rosa26‐LacZ mice were obtained from the Jackson Laboratory. These two mouse strains were crossed to generate the Lgr5 EGFP‐Ires‐CreERT2; Rosa26‐LacZ mice. All animals were housed in a facility with a 12‐h light/12‐h dark cycle.

H pylori colonize and manipulate the progenitor and stem cell compartments, which alters turnover kinetics and glandular hyperplasia. Bacterial ability to alter the stem cells has important implications for gastrointestinal stem cell biology and H pylori-induced gastric pathology. By using the knock-in allele Lgr5 EGFP-Ires-CreERT2, we have shown that the cell surface receptor Lgr5 (leucine-rich repeat–containing heterotrimeric guanine nucleotide–binding protein–coupled receptor 5) marks normal tissue stem cells in stomach, small intestine, colon, and hair follicles . 2012-09-27 · Lgr5-EGFP-Ires-CreERT2, Lgr5-lacZ, and Rosa26-4color mice were generated as described previously (Barker et al., 2007). Rosa26RlacZ mice were obtained from Jackson Labs. All animal experiments were approved by the Animal Experimentation Committee of the Royal Dutch Academy of Science and the Institutional Animal Care and Use Committee of Singapore. Lgr5-EGFP-ires-creERT2 mice,11 APCﬂ/ﬂ mice,25 Axin2-lacZ mice,26 and C57B/6 wild-type mice (6–12 weeks old) were used for experiments.

Lgr5-egfp-ires-creert2

Se hela listan på genoway.com Lgr5-EGFP-IRES-CreERT2 mouse small intestine kindly provided by Omer Yilmaz of MIT, Cambridge, MA. Immunohistochemical analysis of paraffin-embedded Villin-CreERT2 mouse small intestine (left) and wild type mouse small intestine (right) using Cre Recombinase (D7L7L) XP ® Rabbit mAb. Lgr5EGFP-Ires-CreERT2/Apc fl/flmice with the R26R-Confetti strain. Upon stochastic Cre induction by low-dose tamoxifen in adult mice, intestinal ade-nomas developed from Apc-deleted Lgr5+ stem cells.After4weeks,weobservedlargeadenomas (fig. S2, A to C) that either uniformly expressed a single Confetti colororconsistedofanatomically 2020-06-10 · (n = 1 with 3 replicates for WT, all C3(1)-Tag, and healthy MMTV-PyMT; n = 2 MMTV-PyMT for Lgr4, Lgr6, and Lgr5 carcinoma, n = 4 MMTV-PyMT for Lgr5 hyperplasia and adenoma) b Tamoxifen (Tam)-induced Cre recombinase knocked into the Lgr5 locus, Lgr5-EGFP-IRES-creERT2 crossed to Tomato reporter mice, Ai14(R26R dtTomato) indicated Lgr5 + cell progeny in hyperplasias (whole mounts, 1-week Tam observed in Lgr5-LacZ and Lgr5EGFP-ires-CreERT2 knock-in mouse models established later [15]. Then, a lineage tracing experiment in Lgr5EGFP-ires-CreERT2/R26R-LacZ mice identi-fied Lgr5 to be a reliable marker for intestinal stem cells.

2021-01-15 · Lgr5-eGFP-IRES-CreERT2 mice were crossed with B6.Cg-Gt (ROSA)26Sortm9(CAG-tdTomato)Hze/J mice (Jackson Laboratories) to generate Lgr5-eGFP-IRES-CreERT2; Rosa26-CAG-tdTomato heterozygote. Involvement Lgr5 RSCs in rectal epithelial regeneration was examined by lineage tracing assay.

To label only Lgr5 stem cells, Lgr5-EGFP-IRES-CreERT2/Rosa-LSL-tdTomato mouse was injected with. For genetic lineage tracing, Lgr5-EGFP-IRES-cre/ERT2 mice were crossed with Rosa26-tdTomato mice. To induce the expression of the reporter gene, a single We crossed Lgr5-EGFP-IRES-CreERT2 knock-in and ROSA26-tdTomato (LRT) mice, and treated them with or without 4-hydroxytamoxifen (4-OHT), to determine Aug 1, 2012 To study the behavior of Lgr5+ cells within Apc-mutant adenomas, we then crossed Lgr5EGFP-Ires-CreERT2/Apcfl/fl mice with the R26R-Confetti Exogenous IFN-γ depletes Paneth cells and modifies the expression of rapidly cycling ISC markers.

2021-01-15 · Lgr5-eGFP-IRES-CreERT2 mice were crossed with B6.Cg-Gt (ROSA)26Sortm9(CAG-tdTomato)Hze/J mice (Jackson Laboratories) to generate Lgr5-eGFP-IRES-CreERT2; Rosa26-CAG-tdTomato heterozygote. Involvement Lgr5 RSCs in rectal epithelial regeneration was examined by lineage tracing assay.

Experiment Expression of LGR5, an Intestinal Stem Cell Marker, During Lgr5+ve Stem Cells Drive Self-Renewal in the Stomach and . Lgr5 gene · Lgr5 antibody · Lgr5+ intestinal stem cells · Lgr5-egfp-ires-creert2 · Lgr5 wnt · Lgr5620kq1 · Lgr5 protein Lgr5-EGFP-IRES- creERT2 knock-in-möss matades dieter innehållande n-6 PUFA (kontroll), n-3 PUFA, n-6 PUFA + curcumin eller n-3 PUFA + Lgr5-EGFP-IRES-CreERT2; Rosa26-floxed-tdTomato-möss användes för att generera nya data för uppskattning av klonala expansionsparametrar i pylorkörtlar Vi har alla Lgr5 Samling av foton. Lgr5. Lgr5 Gene Lgr5 Antibody. Hemsida. Expression of Lgr5 protein in the human endometrium. Lgr5 gene · Lgr5 antibody · Lgr5+ intestinal stem cells · Lgr5-egfp-ires-creert2 · Lgr5 wnt · Lgr5620kq1 och linjestudier med Lgr5-EGFP-IRES-Creert2- knock-in-möss och Rosa26-LacZ- Liksom i Lgr5 DTR-EGFP / + -knock-musen höll homeostas i den ympade För att undersöka om ARTS kan användas för Lgr5 + ISC-underhåll, använde vi Lgr5-EGFP-IRES-creert2 (betecknade Lgr5 EGFP ) konstitutiva reportermöss, PUMA + / + och PUMA - / - kullkamrater på C57BL / 6-bakgrund (F10) 48 och Lgr5-EGFP ( Lgr5-EGFP-IRES-creERT2 ) -möss 25 har beskrivits.

2018-01-09 · Lgr5-EGFP-Ires-CreERT2;R26R-Confetti double transgenic mice were treated with vehicle (n = 8) or G007-LK (n = 5) and tamoxifen according to the two administration schemes outlined in Fig. 1d, f. In control mice, lineage traced cells populated the entire length of the villi (Fig. 2 a) in the majority (69.9%) of the villi (Additional file 3 : Figure S2A).
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在线客服 ; 实验动物一站式采购平台 cells in Lgr5-eGFP-IRES-CreERT2 mice.

We compared Lrig1 and Lgr5 expression, as Lgr5 is an established SC 2 Feb 2018 The regenerative response of Lgr5-positive ISC was examined by lineage tracing assay using Lgr5-EGFP-ires-CreERT2-TdT mice with Vav3−/− (Lgr5 APC V2V3) and Lgr5-EGFP-IRES-creERT2 Apcfl/fl.
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och linjestudier med Lgr5-EGFP-IRES-Creert2- knock-in-möss och Rosa26-LacZ- Liksom i Lgr5 DTR-EGFP / + -knock-musen höll homeostas i den ympade

All animals were housed in a facility with a 12‐h light/12‐h dark cycle. Although small and large intestines possess seemingly similar Wnt-driven leucine-rich repeat-containing G protein–coupled receptor 5 (Lgr5)+ adult epithelial stem cells, we report here that the two organs exhibit distinct mechanisms of tissue response to ionizing radiation.

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(A) GFP imaging (green) plus Immunofluorescence staining for lysozyme (red) in Tissues from Lgr5-EGFP-IRES-CreERT2 Apcfl/fl mice (bottom row of panels),

In this mouse model, GFP expression is driven from the Lgr5 locus, leading to highest GFP levels in Lgr5 + cells (GFP high). pressesLgr5-EGFPintheLgr5EGFP-Ires-CreERT2knock-inmice.The results showed that Lgr5-EGFP cells did not express the HBC markers, K14 and ICAM-1 (Fig. 1D,E).

View Lgr5 allele: origin, molecular description, gene associations, and references.

Hemsida. Expression of Lgr5 protein in the human endometrium. Lgr5 gene · Lgr5 antibody · Lgr5+ intestinal stem cells · Lgr5-egfp-ires-creert2 · Lgr5 wnt · Lgr5620kq1 och linjestudier med Lgr5-EGFP-IRES-Creert2- knock-in-möss och Rosa26-LacZ- Liksom i Lgr5 DTR-EGFP / + -knock-musen höll homeostas i den ympade För att undersöka om ARTS kan användas för Lgr5 + ISC-underhåll, använde vi Lgr5-EGFP-IRES-creert2 (betecknade Lgr5 EGFP ) konstitutiva reportermöss, PUMA + / + och PUMA - / - kullkamrater på C57BL / 6-bakgrund (F10) 48 och Lgr5-EGFP ( Lgr5-EGFP-IRES-creERT2 ) -möss 25 har beskrivits. Möss utsattes för I botten av krypterna ligger Lgr5 + intestinala stamceller (ISCs) mellan från Lgr5-GFP-IRES-CreERT2- möss återuppslammades i det ENR-innehållande För att undersöka ytterligare använde vi Lgr5-EGFP-Ires-CREERT2- knockinmusen (hädanefter benämnd Lgr5CRE ER ), där den endogena Lgr5- promotorn På grund av den tidigare rapporterade låga aktiviteten hos Lgr5-Cre ERT2 Eftersom Lgr5-eGFP-IRES-CreERT2 knock-in-möss bar en förbättrad grön 3i) och Lgr5 EGFP-IRES-CreERT2- möss som användes i den sporadiska karcinogenesmodellen (fig. 3p) undersöktes för EGFP-uttryck som indikerar Lgr5 + While homozygous mice are not viable, heterozygous Lgr5-EGFP-IRES-CreERT2 mice are viable and fertile; harboring a Lgr5-EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 (Gpr49) gene function and expresses EGFP and CreERT2 fusion protein from the Lgr5 promoter/enhancer elements. A EGFP-IRES-creERT2-WPRE-polyA expression cassette was knocked into the Lgr5 gene start codon site via CRISPR/Cas9 mediated recombination.